Publications

2026

Comparing Trigeminal Nerve Branches in Lampreys and Gnathostomes Reveals Evolutionary Changes in Vertebrate Oral Nerve Innervation

Nakanishi Y, Tamura M, Sugahara F, Kawada N, Ishikawa R, Yamakami S, Maruyama-Watanabe A, Pascual-Anaya J, Kuraku S, Saitou T, Fukui M, Koyama H, Kuratani S, Rijli FM, Suzuki DG, Murakami Y.

Journal of Comparative Neurology 534(3): e70154.

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2024

Hagfish genome elucidates vertebrate whole-genome duplication events and their evolutionary consequences

Yu D, Ren Y, Uesaka M, Beavan AJS, Muffato M, Shen J, Li Y, Sato I, Wan W, Clark JW, Keating JN, Carlisle EM, Dearden RP, Giles S, Randle E, Sansom RS, Feuda R, Fleming JF, Sugahara F, Cummins C, Patricio M, Akanni W, D'Aniello S, Bertolucci C, Irie N, Alev C, Sheng G, de Mendoza A, Maeso I, Irimia M, Fromm B, Peterson KJ, Das S, Hirano M, Rast JP, Cooper MD, Paps J, Pisani D, Kuratani S, Martin FJ*, Wang W*, Donoghue PCJ*, Zhang YE*, Pascual-Anaya J*.

Nature Ecology & Evolution 8(3): 519–535.

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2023

Comparative analysis of Hmx expression and the distribution of neuronal somata in the trigeminal ganglion in lamprey and shark: insights into the homology of the trigeminal nerve branches and the evolutionary origin of the vertebrate jaw

Tamura M, Ishikawa R, Nakanishi Y, Pascual-Anaya J, Fukui M, Saitou T, Sugahara F, Rijli FM, Kuratani S, Suzuki DG, Murakami Y.

Zoological Letters 9(1): 23.

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2023

Evolution of Cyclostome Hox Clusters

Pascual-Anaya J*, Böhmer C.

In Hox Modules in Evolution and Development, Ferrier DEF, ed. CRC Press, pp. 121–.

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2023

High-quality total RNA extraction from early-stage lamprey embryos

Sugahara F, Pascual-Anaya J.

Biotechniques 74(5): 243–278.

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2022

Editorial: New Approaches in Chordate and Vertebrate Evolution and Development

Pascual-Anaya J*, D'Aniello S*, Bertrand S*.

Frontiers in Cell and Developmental Biology 10: 917101.

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2022

Evolution of the nitric oxide synthase family in vertebrates and novel insights in gill development

Annona G, Sato I, Pascual-Anaya J, Osca D, Braasch I, Voss R, Stundl J, Soukup V, Ferrara A, Fontenot Q, Kuratani S, Postlethwait JH, D'Aniello S.

Proceedings of the Royal Society B: Biological Sciences 289(1980): 20220667.

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2022

Thyroid and endostyle development in cyclostomes provide new insights into the evolutionary history of vertebrates

Takagi W, Sugahara F, Higuchi S, Kusakabe R, Pascual-Anaya J, Sato I, Oisi Y, Ogawa N, Miyanishi H, Adachi N, Hyodo S, Kuratani S.

BMC Biology 20(1): 76.

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2021

Forebrain Architecture and Development in Cyclostomes, with Reference to the Early Morphology and Evolution of the Vertebrate Head

Sugahara F, Murakami Y, Pascual-Anaya J, Kuratani S.

Brain Behaviour and Evolution 96(4–6): 305–317.

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2021

Genetic Mechanism for the Cyclostome Cerebellar Neurons Reveals Early Evolution of the Vertebrate Cerebellum

Sugahara F, Pascual-Anaya J, Kuraku S, Kuratani S, Murakami Y.

Frontiers in Cell and Developmental Biology 9: 700860.

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2019

How do morphological novelties evolve? Novel approaches to define novel morphologies

Almudí I*, Pascual-Anaya J*.

In Old Questions and Young Approaches to Animal Evolution, Martín-Durán JM and Vellutini BC, eds. Springer, pp. 107–132.

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2019

Inner ear development in cyclostomes and evolution of the vertebrate semicircular canals

Higuchi S, Sugahara F, Pascual-Anaya J, Takagi W, Oisi Y, Kuratani S.

Nature 565(7739): 347–350.

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2018

A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins

Letelier J, de la Calle-Mustienes E, Pieretti J, Naranjo S, Maeso I, Nakamura T, Pascual-Anaya J, Shubin NH, Schneider I, Martinez-Morales JR, Gómez-Skarmeta JL.

Nature Genetics 50(4): 504–509.

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2018

Development of hypobranchial muscles with special reference to the evolution of the vertebrate neck

Adachi N, Pascual-Anaya J, Hirai T, Higuchi S, Kuratani S.

Zoological Letters 4: 5.

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2018

Hagfish and lamprey Hox genes reveal conservation of temporal colinearity in vertebrates

Pascual-Anaya J*, Sato I, Sugahara F, Higuchi S, Paps J, Ren Y, Takagi W, Ruiz-Villalba A, Ota KG, Wang W, Kuratani S.

Nature Ecology & Evolution 2(5): 859–866.

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2018

Stepwise participation of HGF/MET signaling in the development of migratory muscle precursors during vertebrate evolution

Adachi N, Pascual-Anaya J, Hirai T, Higuchi S, Kuroda S, Kuratani S.

Zoological Letters 4: 18.

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2017

Molecular regionalization of the developing amphioxus neural tube challenges major partitions of the vertebrate brain

Albuixech-Crespo B, López-Blanch L, Burguera D, Maeso I, Sánchez-Arrones L, Moreno-Bravo JA, Somorjai I, Pascual-Anaya J, Puelles E, Bovolenta P, Garcia-Fernàndez J, Puelles L, Irimia M, Ferran JL.

PLOS Biology 15(4): e2001573.

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2017

Nitric Oxide regulates mouth development in amphioxus

Annona G, Caccavale F, Pascual-Anaya J, Kuratani S, De Luca P, Palumbo A, D'Aniello S.

Scientific Reports 7(1): 8432.

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2017

Reconstructing the ancestral vertebrate brain

Sugahara F, Murakami Y, Pascual-Anaya J, Kuratani S.

Development Growth & Differentiation 59(4): 163–174.

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2016

Evidence from cyclostomes for complex regionalization of the ancestral vertebrate brain

Sugahara F, Pascual-Anaya J, Oisi Y, Kuraku S, Aota S, Adachi N, Takagi W, Hirai T, Sato N, Murakami Y, Kuratani S.

Nature 531(7592): 97–100.

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2015

The Evolutionary Origin of the Turtle Shell and Its Dependence on the Axial Arrest of the Embryonic Rib Cage

Hirasawa T, Pascual-Anaya J, Kamezaki N, Taniguchi M, Mine K, Kuratani S.

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 324(3): 194–207.

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2014

Comparative analysis of pleurodiran and cryptodiran turtle embryos depicts the molecular ground pattern of the turtle carapacial ridge

Pascual-Anaya J*, Hirasawa T, Sato I, Kuraku S, Kuratani S*.

International Journal of Developmental Biology 58(10–12): 743–750.

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2013

Evolution of Hox gene clusters in deuterostomes

Pascual-Anaya J*, D'Aniello S, Kuratani S, Garcia-Fernàndez J*.

BMC Developmental Biology 13: 26.

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2013

The draft genomes of soft-shell turtle and green sea turtle yield insights into the development and evolution of the turtle-specific body plan

Wang Z, Pascual-Anaya J [co-first author] et al.

Nature Genetics 45(6): 701–706.

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2013

The evolutionary origins of chordate hematopoiesis and vertebrate endothelia

Pascual-Anaya J*, Albuixech-Crespo B, Lara Somorjai IM, Carmona R, Oisi Y, Alvarez S, Kuratani S, Muñoz-Chápuli R, Garcia-Fernàndez J*.

Developmental Biology 375(2): 182–192.

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2012

Broken colinearity of the amphioxus Hox cluster

Pascual-Anaya J, Adachi N, Alvarez S, Kuratani S, D'Aniello S, Garcia-Fernàndez J.

EvoDevo 3(1): 28.

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2010

Cis-regulatory characterization of sequence conservation surrounding the Hox4 genes

Punnamoottil B, Herrmann C, Pascual-Anaya J, D'Aniello S, Garcia-Fernàndez J, Akalin A, Becker TS, Rinkwitz S.

Developmental Biology 340(2): 269–282.

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2009

From the American to the European amphioxus: towards experimental Evo-Devo at the origin of chordates

Garcia-Fernàndez J, Jiménez-Delgado S, Pascual-Anaya J, Maeso I, Irimia M, Minguillón C, Benito-Gutiérrez E, Gardenyes J, Bertrand S, D'Aniello S.

International Journal of Developmental Biology 53(8–10): 1359–1366.

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2009

Implications of duplicated cis-regulatory elements in the evolution of metazoans: the DDI model or how simplicity begets novelty

Jiménez-Delgado S, Pascual-Anaya J, Garcia-Fernàndez J.

Briefings in Functional Genomics 8(4): 266–275.

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2008

Gene expansion and retention leads to a diverse tyrosine kinase superfamily in amphioxus

D'Aniello S, Irimia M, Maeso I, Pascual-Anaya J, Jiménez-Delgado S, Bertrand S, Garcia-Fernàndez J.

Molecular Biology and Evolution 25(9): 1841–1854.

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2008

The amphioxus Hox cluster: Characterization, comparative genomics, and evolution

Amemiya CT, Prohaska SJ, Hill-Force A, Cook A, Wasserscheid J, Ferrier DEK, Pascual-Anaya J, Garcia-Fernàndez J, Dewar K, Stadler PF.

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 310(5): 465–477.

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2008

Unexpectedly large number of conserved noncoding regions within the ancestral chordate Hox cluster

Pascual-Anaya J, D'Aniello S, Garcia-Fernàndez J.

Development Genes and Evolution 218(11–12): 591–597.

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2007

Insights into spawning behavior and development of the European amphioxus (Branchiostoma lanceolatum)

Fuentes M, Benito E, Bertrand S, Paris M, Mignardot A, Godoy L, Jimenez-Delgado S, Oliveri D, Candiani S, Hirsinger E, D'Aniello S, Pascual-Anaya J, Maeso I, Pestarino M, Vernier P, Nicolas JF, Schubert M, Laudet V, Geneviere AM, Albalat R, Garcia Fernandez J, Holland ND, Escriva H.

Journal of Experimental Zoology Part B: Molecular and Developmental Evolution 308(4): 484–493.

PubMed →

2006

Free amino acids in the nervous system of the amphioxus Branchiostoma lanceolatum. A comparative study

Pascual-Anaya J, D'Aniello S.

International Journal of Biological Sciences 2(2): 87–92.

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